Identification of the transgene insertion site for an adipocyte-specific adiponectin-cre model and characterization of the functional consequences

The Adipoq-Cre transgenic mouse is widely used in the development of adipocyte-specific genetic manipulations for the study of obesity and type 2 diabetes. In the process of developing a new mouse model utilizing the adipocyte selective Adipoq-Cre transgenic mouse, strong genetic linkage between a gene of interest, Adam10, and the Adipoq-Cre transgene was discovered. Whole-genome sequencing of the Adipoq-Cre transgenic mouse model identified the genomic insertion site within the Tbx18 gene locus on chromosome 9 and this insertion causes a significant decrease in Tbx18 gene expression in adipose tissue. Insertion of genes Kng2, Kng1, Eif4a2 and Rfc4 also occurred in the Adipoq-Cre transgenic mouse, and these passenger genes may have functional consequences in various tissues.

Automated summary

The Adipoq-Cre transgenic mouse is commonly used in obesity and type 2 diabetes research, with a strong genetic linkage to the Adipoq-Cre transgene. The genomic insertion site was identified within the Tbx18 gene locus on chromosome 9, causing a significant decrease in Tbx18 gene expression in adipose tissue. Additional genes Kng2, Kng1, Eif4a2, and Rfc4 were also inserted in the Adipoq-Cre transgenic mouse, potentially impacting various tissues.