4.7 Article

A Broccoli aptamer chimera yields a fluorescent K+ sensor spanning physiological concentrations

Journal

CHEMICAL COMMUNICATIONS
Volume 57, Issue 11, Pages 1344-1347

Publisher

ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc07042d

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By creating a chimera of Broccoli RNAs with mixed chemistries, it is possible to develop an effective RNA-based fluorescent K+ sensor that maintains structure and fluorescence properties without interference, providing linear fluorescent gain across physiological K+ concentrations.
The RNA aptamer Broccoli accepts 2 ' fluorinated (2 ' F) pyrimidine nucleotide incorporation without perturbation of structure or fluorescence in the presence of potassium and DFHBI. However, the modification decreases Broccoli's apparent affinity for K+ >30-fold. A chimera of Broccoli RNAs with mixed chemistries displays linear fluorescent gain spanning physiological K+ concentrations, yielding an effective RNA-based fluorescent K+ sensor.

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