Journal
FUTURE MICROBIOLOGY
Volume 12, Issue 9, Pages 781-799Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2016-0230
Keywords
glycosidases; mucinases; mucins; Naegleria fowleri; secretory products
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Funding
- CONACyT [237523]
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Aim: The aim of this work was to identify, characterize and evaluate the pathogenic role of mucinolytic activity released by Naegleria fowleri. Materials & methods: Zymograms, protease inhibitors, anion exchange chromatography, MALDI-TOF-MS, enzymatic assays, Western blot, and confocal microscopy were used to identify and characterize a secreted mucinase; inhibition assays using antibodies, dot-blots and mouse survival tests were used to evaluate the mucinase as a virulence factor. Results: A 94-kDa protein with mucinolytic activity was inducible and abolished by p-hydroxymercuribenzoate. MALDI-TOF-MS identified a glycoside hydrolase. Specific antibodies against N. fowleri-glycoside hydrolase inhibit cellular damage and MUC5AC degradation, and delay mouse mortality. Conclusion: Our findings suggest that secretory products from N. fowleri play an important role in mucus degradation during the invasion process.
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