Journal
FUTURE MICROBIOLOGY
Volume 12, Issue 16, Pages 1449-1455Publisher
FUTURE MEDICINE LTD
DOI: 10.2217/fmb-2016-0195
Keywords
acute gastroenteritis; diarrhea; Escherichia coli; gelatin tannate; inactivated probiotics; mucoprotectors
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Funding
- Novintethical, SA
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Aim: To compare the protective efficacy of gelatine tannate/probiotic with other antidiarrheal agents in Escherichia coli-inoculated CacoGoblet (R) cells. Methods: Four test compounds - gelatine tannate plus inactivated probiotic, diosmectite, probiotic mixture and Saccharomyces boulardii - were added to E. coli-infected CacoGoblet cells. After 1 and 24 h, transepithelial electrical resistance was measured and a lucifer yellow assay performed. Results: Gelatine tannate/probiotic markedly increased transepithelial electrical resistance by 123.1% (at 1 h) and 149.5% (at 24 h), and produced paracellular flux values of 0.41% (1 h) and 1.34% (24 h), which were considerably less than the E. coli-invasion value (2.41%). Conclusion: The protective efficacy of gelatine tannate/probiotic against E. coli-induced reduction of membrane integrity manifests early and is maintained for 24 h.
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