Journal
BIOFOULING
Volume 37, Issue 1, Pages 117-130Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/08927014.2021.1885649
Keywords
Biofilm; Candida albicans; denture cleansers; denture stomatitis; Lippia
Funding
- National Council for Scientific and Technological Development [444449/2014-0, MCTI/CNPq 14/2014]
- Conselho Nacional de Desenvolvimento Cientifico e Tecnologico
- Coordination for the Improvement of Higher Level or Education Personnel (CAPES Brazil)
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The experimental solution containing Lippia sidoides essential oil showed antimicrobial activity against different pathogens, reduced the metabolic activity of C. albicans biofilm significantly, and caused cell wall damage at low concentrations, making it a potential alternative for the prevention and treatment of denture-induced infections.
The antimicrobial activity of an experimental solution containing essential oil of Lippia sidoides for denture cleaning was evaluated by (1) minimum inhibitory (MIC) and fungicidal/bactericidal concentration (MFC/MBC) tests against Candida albicans, Staphylococcus aureus, and Pseudomona aeruginosa; (2) the metabolic activity of C. albicans biofilm formed on flat-bottom microplates and denture base specimens based on the reduction of 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT); and (3) scanning electron microscopy, to evaluate the fungal biofilm morphology. The solution showed antimicrobial action against the pathogens tested (C. albicans - MIC and MFC: 19.53 mu g ml(-1), S. aureus - MIC and MBC: 78.12 mu g ml(-1), P. aeruginosa - MIC: 625 mu g ml(-1), MBC: 2,500 mu g ml(-1)), reduced the metabolic activity of C. albicans biofilm up to 97%, and caused cell wall damage at low concentrations (195.3-390.6 mu g ml(-1)) and in short time periods (20 min). Therefore, the experimental solution has the potential to be used as an alternative in the prevention and treatment of denture-induced infections.
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