Journal
MARINE POLLUTION BULLETIN
Volume 162, Issue -, Pages -Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.marpolbul.2020.111841
Keywords
Microplastic; Marine sediments; Optimization; Plackett-Burman; Factorial design
Funding
- Spanish Inter-Ministerial Science and Technology Commission through the IMPACTA project (CICYT) [CTM2013-48194-C3-1-R]
- Spanish Inter-Ministerial Science and Technology Commission through the BASEMAN project [PCIN-2015170-CO2-02]
- Spanish Inter-Ministerial Science and Technology Commission through the 2-2 ESMARME project
- European Union through the European Regional Development Fund (ERDF)
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This study optimized the procedure for extracting microplastics from marine sediments, with the Plackett-Burman design revealing sediment mass and agitation time as the most significant variables.
Microplastics are gaining worldwide attention due to their omnipresence. The marine environment is one of the most affected systems; especially the sediment compartment. Microplastic separation from the sediment matrix is the first step to evaluate its abundance and availability. Nevertheless, a lack of consistency in extraction protocols is a fact. This paper describes the optimization of the microplastic extraction procedure from marine sediments. The Plackett-Burman saturated factorial design was used to identify the significant factors and to select optimum working conditions. With this purpose, the following variables were studied: the number of extractions; the amount of sediment; the settling time; the density separation solution volume; the agitation time and the suitability of using wet or freeze-dried sediment. The Plackett-Burman design has revealed that the most statistically significant variables were sediment mass and agitation time. The optimized method was applied for two marine sediments collected in the Mar Menor Lagoon.
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