Journal
VETERINARY SCIENCES
Volume 8, Issue 2, Pages -Publisher
MDPI
DOI: 10.3390/vetsci8020024
Keywords
feline; Wharton’ s jelly; mesenchymal stem cell; characterization
Categories
Funding
- National Research Foundation of Korea (NRF) - Korea government (MSIT) [NRF-2017R1C1B5018221]
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Wharton's jelly is a known source of mesenchymal stem cells in various species, but there have been no reports on cats. This study isolated and characterized mesenchymal stem cells from the Wharton's jelly of cats, demonstrating their differentiation potential and therapeutic applications in feline medicine.
Wharton's jelly is a well-known mesenchymal stem cell source in many species, including humans. However, there have been no reports confirming the presence of mesenchymal stem cells in Wharton's jelly in cats. The purpose of this study was to isolate mesenchymal stem cells (MSCs) from the Wharton's jelly of cats and to characterize stem cells. In this study, feline Wharton's jelly-derived mesenchymal stem cells (fWJ-MSCs) were isolated and successfully cultured. fWJ-MSCs were maintained and the proliferative potential was measured by cumulative population doubling level (CPDL) test, scratch test, and colony forming unit (CFU) test. Stem cell marker, karyotyping and immunophenotyping analysis by flow cytometry showed that fWJ-MSCs possessed characteristic mesenchymal stem cell markers. To confirm the differentiation potential, we performed osteogenic, adipogenic and chondrogenic induction under each differentiation condition. fWJ-MSCs has the ability to differentiate into multiple lineages, including osteogenic, adipogenic and chondrogenic differentiation. This study shows that Wharton's jelly of cat can be a good source of mesenchymal stem cells. In addition, fWJ-MSCs may be useful for stem cell-based therapeutic applications in feline medicine.
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