Journal
HELIYON
Volume 7, Issue 1, Pages -Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.heliyon.2021.e06085
Keywords
Thraustochytrids; Fructose; Factorial design; Docosahexaenoic acid (DHA)
Categories
Funding
- MINISTRY OF HIGHER EDUCATION MALAYSIA under the Fundamental Research Grant Scheme [FRGS/2/2013/SG05/UKM/02/1]
Ask authors/readers for more resources
Fructose has a significant positive effect on DHA content in Thraustochytrids, with a less profound impact on DHA biosynthetic capacity. When cultivated in fructose medium, Thraustochytrid species exhibit a slower growth rate but higher biomass and lipid accumulation compared to glucose medium after 48 hours.
Thraustochytrids are getting increasingly popular due to their high potential role as alternative producers of the high-valued omega-3 polyunsaturated fatty acids (PUFA), docosahexaenoic acid (DHA). While most thraustochytrids prefer glucose as the major carbon source, few strains have been reported to prefer fructose. One such strain is Aurantiochytrium sp. SW1. In this study, the effect of fructose on DHA accumulation by SW1 was investigated using a two-level full factorial design. Besides, biomass, lipid and DHA accumulation profiles of SW1 cultivated in fructose and glucose media were compared. Results revealed that fructose has a very significant positive effect on the volumetric DHA content. Meanwhile, its involvement in affecting DHA biosynthetic capacity, though significant, is not very profound. It was also found that when cultivated in fructose medium, SW1 had a less steep log phase compared to that of glucose medium. However, after 48h of cultivation, biomass and lipid accumulation in fructose medium outweighed the other. Volumetric DHA content in fructose medium at 96h was 11% higher than that of glucose medium. Overall, fructose was found to be a more suitable substrate for biomass, lipid and DHA accumulation in SW1 compared to the conventional source, glucose.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available