Journal
AGING-US
Volume 13, Issue 1, Pages 769-781Publisher
IMPACT JOURNALS LLC
Keywords
microRNA; proliferation; metastasis; TMEM98; head and neck squamous cell carcinoma
Categories
Funding
- Science and Technology Plan Projects of Foshan [2018AB003241]
- Natural Science Foundation of Guangdong Province [2016A030313245]
- Special fund of Foshan Summit [2019C017]
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This study identified miR-29c-5p as a potential therapeutic target for managing HNSCC by inhibiting cell migration and proliferation. The effects of miR-29c-5p on HNSCC tumors were confirmed using a mouse tumor xenograft model. TMEM98 was identified as a direct target of miR-29c-5p in HNSCC cells.
Head and neck squamous cell carcinoma (HNSCC), which occurs frequently worldwide, is characterized by high risk of metastasis. MicroRNAs (miRNAs) play crucial roles in tumorigenesis and cancer development. In this study, miR-29c-5p was identified using three high throughput microarrays. We measure miR-29c-5p expression in HNSCC tissues and cell lines. To determine the function of miR-29c-5p in HNSCC, we evaluated its effects in vitro on cell proliferation, the cell cycle, apoptosis, and cell migration. We employed a mouse tumor xenograft model to determine the effects of miR-29c-5p on tumors generated by HNSCC cell lines. The miR-29c-5p expression was lower in HNSCC tissues than in normal tissues. Upregulated miR-29c-5p expression in HNSCC cells inhibited migration and arrested cells in the G2/M phase of the cell cycle. Further, upregulated miR-29c-5p expression inhibited the proliferation of HNSCC cells in vivo and in vitro. In addition, transmembrane protein 98 (TMEM98) was identified as a direct target of miR-29c-5p by using a luciferase reporter assay. These findings provide new insights that link the regulation of miR-29c-5p expression to the malignant phenotype of HNSCC and suggest that employing miR-29c-5p may serve as a therapeutic strategy for managing patients with HNSCC.
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