4.5 Article

Localization and regulation of claudin-14 in experimental models of hypercalcemia

Journal

AMERICAN JOURNAL OF PHYSIOLOGY-RENAL PHYSIOLOGY
Volume 320, Issue 1, Pages F74-F86

Publisher

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajprenal.00397.2020

Keywords

calcium-sensing receptor; claudin-14; hypercalciuria; kidney; thick ascending limb

Funding

  1. Fabrikant Vilhelm Pedersen og Hustrus Mindelegat
  2. Novo Nordisk Foundation
  3. Beckett Foundation
  4. Carlsberg Foundation
  5. Independent Research Fund Denmark

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CLDN14 has a restricted localization pattern in the apical domain of select cells of the TAL, mainly co-expressed with CLDN16.
Variations in the claudin-14 (CLDN14) gene have been linked to increased risk of hypercalciuria and kidney stone formation. However, the exact cellular localization of CLDN14 and its regulation remain to be fully delineated. To this end, we generated a novel antibody that allowed the detection of CLDN14 in paraffin-embedded renal sections. This showed CLDN14 to be detectable in the kidney only after induction of hypercalcemia in rodent models. Protein expression in the kidney is localized exclusively to the thick ascending limbs (TALs), mainly restricted to the cortical and upper medullary portion of the kidney. However, not ail cells in the TALs expressed the tight junction protein. In fact, CLDN14 was primarily expressed in cells also expressing CLDN16 but devoid of CLDN10. CLDN14 appeared in very superficial apical cell domains and near cell junctions in a belt-like formation along the apical cell periphery. In transgenic mice, Cldn14 promotor-driven LacZ activity did not show complete colocalization with CLDN14 protein nor was it increased by hypercalcemia, suggesting that LacZ activity cannot be used as a marker for CLDN14 localization and regulation in this model. In conclusion, CLDN14 showed a restricted localization pattern in the apical domain of select cells of the TAL.

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