Journal
MATERIALS CHEMISTRY FRONTIERS
Volume 5, Issue 4, Pages 1872-1883Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0qm00682c
Keywords
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Funding
- National Natural Science Foundation of China [21975197, 21674085, 51603165]
- Young Talent Fund of University Association for Science and Technology in Shaanxi, China [20180601]
- Natural Science Basic Research Plan in Shaanxi Province of China [2019JM-040]
- Key Laboratory Construction Program of Xi'an Municipal Bureau of Science and Technology [201805056ZD7CG40]
- Fundamental Fund of Xi'an Jiao Tong University [xzy022020015]
- School of Materials Science and Engineering, Jiangsu Engineering Laboratory of Light-Electricity-Heat Energy-Converting Materials and Applications [GDRGCS2019001]
- Innovation Capability Support Program of Shaanxi [2018PT-28, 2019PT-05]
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An imaging platform of DTPA-BT-M with aggregation-induced emission (AIE) characteristics was developed for lipid droplets (LDs)-specific super-resolution cellular imaging and deep-penetrated tissue imaging. DTPA-BT-M showed superior fluorescence properties and a high photoluminescence quantum yield, allowing for ultra-high resolution imaging of LDs via STED nanoscopy and deep tissue penetration in BALB/C nude mice lung tissue via TPF microscopy.
To obtain lipid droplets (LDs)-specific super-resolution cellular imaging and deep-penetrated tissue imaging, an imaging platform of DTPA-BT-M with aggregation-induced emission (AIE) characteristics for stimulated emission depletion (STED) nanoscopy and two-photon fluorescence (TPF) microscopy was developed here. Benefiting from its excellent AIE properties, superior fluorescence properties in a deep-red region are observed for DTPA-BT-M with a high photoluminescence quantum yield of up to 33.96% in the solid state. In addition, DTPA-BT-M also exhibits large Stokes' shift, outstanding photostability, excellent biocompatibility and high LD specificity. Furthermore, a large two-photon absorption cross section of up to 1581 GM is also achieved in DTPA-BT-M due to its symmetrical D-A-D architecture. Therefore, the developed AIE luminogen is simultaneously applied in STED nanoscopy and TPF microscopy, leading to an ultra-high resolution (full width at half maximum value, FWHM = 95 nm) in DTPA-BT-M-stained LDs via STED nanoscopy and also a much deep penetration of similar to 300 mu m for lung tissue in BALB/C nude mice by TPF microscopy. The results here indicate that the easily synthesized DTPA-BT-M can be an impressive imaging platform for LDs-specific super-resolution cell imaging and two-photon tissue imaging, thus providing the possibility to further understand LDs' roles in the metabolic process in biological research.
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