Journal
ORGANIC & BIOMOLECULAR CHEMISTRY
Volume 19, Issue 6, Pages 1292-1295Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ob02422h
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Funding
- National Science Center (NCN) of Poland [2020/04/X/ST4/00418]
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The study demonstrates a highly efficient DNA duplex interstrand photocrosslinking method using ClSdU, which forms a reversible crosslink with thymine in the target strand and converts to a stable fluorescent crosslink. The results indicate the great potential of ClSdU as a valuable DNA photo-crosslinking reagent for chemical biology applications.
We describe highly efficient interstrand photocrosslinking of a DNA duplex containing 5-chloro-2 '-deoxy-4-thiouridine (ClSdU) in one strand, proceeding via a two-step photochemical cascade, involving the formation of a thermally reversible crosslink between ClSdU and thymidine in the target strand and its subsequent conversion to a thermally stable fluorescent crosslink. These results show that ClSdU has great potential to be a valuable DNA photo-crosslinking reagent for chemical biology applications.
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