Journal
JOURNAL OF APPLIED ORAL SCIENCE
Volume 29, Issue -, Pages -Publisher
UNIV SAO PAULO FAC ODONTOLOGIA BAURU
DOI: 10.1590/1678-7757-2020-0575
Keywords
Mirror-image pain; Osteoarthritis; Complete Freund adjuvant; Temporomandibular joint; Nocifensive behaviors
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Funding
- Prince of Songkla University Scholarship
- Siriraj Research Fund, Faculty of Medicine Siriraj Hospital, Mahidol University, Thailand [(IO) R016331041]
- Thailand Research Fund through The Royal Golden Jubilee PhD program [PHD 0058/2561]
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By studying mirror-image pain in an osteoarthritis mouse model of the temporomandibular joint, it was found that nocifensive behaviors on the contralateral side became stronger on day 28, in correlation with elevated phosphorylated cyclic AMP response element binding protein and microglia marker in the trigeminal subnucleus caudalis, indicating the occurrence of mirror-image pain.
Mirror-image pain is a kind of pain that occurs on the contralateral side, but its pathogenesis remains unclear. Objective: To develop an osteoarthritis mouse model for investigating mirror-image pain through observing nocifensive behaviors, histological changes, and nociceptive activity at days 3, 7, 14, 21, and 28 after the chemical induction of unilateral temporomandibular joint (TM]) osteoarthritis. Methodology: We randomly divided 6-week-old mice into sham and complete Freund adjuvant groups. To induce nocifensive behaviors, we applied 0.04 g of von Frey filament, 10 psi of air puff, and cold acetone on both sides of whisker pads at different days. The histology of TM3 on both sides was observed by hematoxylin/eosin staining and microcomputed tomography scanning. Furthermore, the nociceptive activity was evaluated using the phosphorylated cyclic AMP response element binding protein (pCREB) and a microglia marker at different days in the trigeminal subnucleus caudalis. Results: Nocifensive behaviors against mechanical and temperature stimuli on the contralateral side became stronger than the baseline on day 28, in agreement with the elevation of the pCREB and the microglia marker in the trigeminal subnucleus caudalis. Thus, hypernociception on the contralateral side occurred at day 28. Conclusions: Clearly, the TM3 model with unilateral osteoarthritis exhibited mirror-image pain. Therefore, this model is useful in investigating the pathogenesis of pain and in developing treatments.
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