Journal
CHEMICAL COMMUNICATIONS
Volume 57, Issue 16, Pages 2041-2044Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cc07763a
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Funding
- National Natural Science Foundation of China [21735003, 21705096]
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We have developed an aptamer-mediated rolling circle amplification approach for the label-free and sensitive detection of histone-modifying enzyme activity. This method can achieve femtomolar sensitivity for the assay of histone acetyltransferase Tip60, and can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
We develop for the first time an aptamer-mediated rolling circle amplification approach for label-free and sensitive detection of histone-modifying enzyme (HME) activity. This method can achieve femtomolar sensitivity for histone acetyltransferase Tip60 assay, which is the most sensitive HME assay reported so far. It can be further applied for inhibitor screening, enzyme kinetic analysis, and endogenous Tip60 measurement in cancer cells.
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