Journal
STAR PROTOCOLS
Volume 2, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100327
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Funding
- NIH [NIGMS 1R01GM127559]
- American Cancer Society [RSG-17-011-01]
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FLICK is a new method that can directly quantify cell death kinetics, simplifying the evaluation of drug sensitivity while retaining high-throughput and broad accessibility.
Evaluating drug sensitivity is improved by directly quantifying death kinetics, rather than correlates of viability, such as metabolic activity. This is challenging, requiring time-lapse microscopy and genetically encoded labels to distinguish live and dead cells. Here, we describe fluorescence-based and lysis-dependent inference of cell death kinetics (FLICK). This method requires only a standard fluorescence plate reader, retaining the high-throughput nature and broad accessibility of common viability assays. However, FLICK specifically quantifies death, including an accurate inference of death kinetics. For complete details on the use and execution of this protocol, please refer to Richards et al. (2020).
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