An optimized protocol for combined fluorescent lectin/ immunohistochemistry to characterize tissue-specific glycan distribution in human or rodent tissues

Lectin histochemical analysis of tissues combined with immunohistochemistry is a valuable tool to characterize and correlate the spatial distribution of gly-cans with the presence of specific cell types or antigens of interest. The cur-rent protocol describes the application of monosaccharide motif specificity of lectin binding to glycan residues to different tissue types. In addition, we describe stereological methods to provide further quantification of the analyzed tissues.For complete details on the use and execution of this protocol, please refer to Mohd Isa et al. (2018), Contessotto et al. (2020), and Samal et al. (2020).

Automated summary

The lectin histochemical analysis of tissues combined with immunohistochemistry is valuable for characterizing and correlating the spatial distribution of glycans with specific cell types or antigens. Stereological methods can also be utilized for further quantification of analyzed tissues.