An updated protocol for the cost-effective and weekend-free culture of human induced pluripotent stem cells

The protocol provided here describes methodologies for making a highly cost-effective, chemically defined medium for culturing hiPSCs we call B8 medium. The typical cost of B8 medium is US$10 per liter, which with modifications included here is more affordable than standard media. We provide simple proto-cols for making B8 supplement aliquots, making the basal media DMEM/F12, Matrigel-coated plates, thawing, passaging, culturing, and cryopreserving hiPSCs. We show typical differentiation results and provide a comprehensive troubleshooting guide.For complete details on the use and execution of this protocol, please refer to Kuo et al. (2020).

Automated summary

The provided protocol details a cost-effective method for culturing hiPSCs using a medium called B8. By offering comprehensive preparation methods and troubleshooting guide, researchers are assisted in conducting cell culture and differentiation experiments more effectively.