Journal
STAR PROTOCOLS
Volume 2, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2020.100254
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Funding
- Creative Research Initiatives Program [NRF-2017R1A3B1023387]
- Science Research Center program [NRF-2016R1A5A1010764]
- Ministry of Education, Culture, Sports, Science, and Technology (MEXT)
- Japan Society for the Promotion of Science (JSPS) KAKENHI grants [JP18K14612, 19H05750]
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Isolation of spermatid fractions using unit gravity sedimentation in a BSA density gradient can be used to analyze changes in transcript or protein expression during spermiogenesis.
Post-meiotic spermatids become spermatozoa through developmental stages during spermiogenesis. Isolation of spermatid fractions is required to examine the change of protein expression during spermiogenesis. Here, we present a sim-ple method to isolate spermatid fractions from mouse testes using unit gravity sedimentation in a BSA density gradient. Isolation of spermatid fractions can be used to analyze changes of transcript or protein during spermiogenesis. For complete details on the use and execution of this protocol, please refer to Kim et al. (2020).
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