Measurement of mitochondrial respiration in adherent cells by Seahorse XF96 Cell Mito Stress Test

Mitochondria play pivotal roles in cellular energy metabolism. Most of the intra-cellular adenosine triphosphate (ATP) is generated by mitochondrial respiration. The Cell Mito Stress Test is a common method to measure the key parameters of mitochondrial respiration. Here, we use the human cell line HK-2 as an example to present the procedures to quantify the oxygen consumption rate using a Sea -horse XFe96 extracellular flux analyzer. For complete details on the use and execution of this protocol, please refer to Ma et al. (2020).

Automated summary

Mitochondria play crucial roles in cellular energy metabolism, generating most of the intracellular ATP through respiration. The Cell Mito Stress Test is a common method for measuring key parameters of mitochondrial respiration, demonstrated here using the human cell line HK-2 as an example.