Measuring microenvironment-tuned nuclear stiffness of cancer cells with atomic force

Quantification of nuclear stiffness is challenging for cells encapsulated within a 3D extracellular matrix (ECM). Here, we describe an experimental setup for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope (AFM). In our setup, ECM-coated polyacrylamide hydro -gels mimic the stiffness of the microenvironment, enabling the measurement of nuclear stiffness using an AFM probe in live cancer cells. For complete details on the use and execution of this protocol, please refer to Das et al. (2019) (https://doi.org/10.1016/j.matbio.2019.01.001).

Automated summary

The experimental setup described in this study allows for measuring microenvironment-dependent tuning of nuclear stiffness using an atomic force microscope in live cancer cells. The use of ECM-coated polyacrylamide hydrogels provides a platform for mimicking the stiffness of the microenvironment, facilitating the measurement of nuclear stiffness. For detailed instructions, refer to Das et al. (2019) (https://doi.org/10.1016/j.matbio.2019.01.001).