Ultra-long DNA molecule isolation from plant nuclei for ultra-long read genome sequencing

Long and ultra-long read DNA sequencing technologies require high molecular weight DNA with high quality and sufficient quantity, which could be challenging to obtain from recalcitrant plant tissues. We describe a protocol to isolate ultra -long DNA from 12 species for ultra-long read genome sequencing. A suitable nuclei lysis buffer is critical for DNA quality and yield. This protocol will enable individual labs to isolate high molecular weight DNA at a rapid pace with low cost from a variety of plant species. For complete information on the use and execution of this protocol, please refer to: Zhang et al. (2020).

Automated summary

The protocol provides detailed instructions on isolating ultra-long DNA from 12 plant species for high-quality genome sequencing, emphasizing the importance of a suitable nuclei lysis buffer. This method enables individual labs to rapidly and cost-effectively obtain high molecular weight DNA from a variety of plant species.