Journal
STAR PROTOCOLS
Volume 2, Issue 1, Pages -Publisher
ELSEVIER
DOI: 10.1016/j.xpro.2021.100343
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Funding
- National Science Foundation (NSF) [DBI-1546890]
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The protocol provides detailed instructions on isolating ultra-long DNA from 12 plant species for high-quality genome sequencing, emphasizing the importance of a suitable nuclei lysis buffer. This method enables individual labs to rapidly and cost-effectively obtain high molecular weight DNA from a variety of plant species.
Long and ultra-long read DNA sequencing technologies require high molecular weight DNA with high quality and sufficient quantity, which could be challenging to obtain from recalcitrant plant tissues. We describe a protocol to isolate ultra -long DNA from 12 species for ultra-long read genome sequencing. A suitable nuclei lysis buffer is critical for DNA quality and yield. This protocol will enable individual labs to isolate high molecular weight DNA at a rapid pace with low cost from a variety of plant species. For complete information on the use and execution of this protocol, please refer to: Zhang et al. (2020).
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