Ionic liquid-assisted chemiluminescent immunoassay of prostate specific antigen using nanoceria as an alkaline phosphatase-like nanozyme label

An alkaline phosphatase-like nanozyme was applied for an immunoassay for the first time. By using nanoceria as the alkaline phosphatase-like catalytic label and CDP-star as the substrate, the chemiluminescent detection of prostate specific antigen was demonstrated. More importantly, the addition of ionic liquid can significantly increase the sensitivity of the immunoassay. With the aid of ionic liquid, an order of magnitude improvement in the sensitivity was achieved with a detection limit of 53 fg mL(-1).

Automated summary

An alkaline phosphatase-like nanozyme was utilized for the first time in an immunoassay, demonstrating chemiluminescent detection of prostate specific antigen with nanoceria as the catalytic label and CDP-star as the substrate. The sensitivity of the immunoassay was significantly increased by the addition of ionic liquid, resulting in an order of magnitude improvement in detection limit to 53 fg mL(-1).