Journal
FREE RADICAL RESEARCH
Volume 51, Issue 5, Pages 517-528Publisher
TAYLOR & FRANCIS LTD
DOI: 10.1080/10715762.2017.1328552
Keywords
Dihydromyricetin; autophagy; NF-kappa B; reactive oxygen species; apoptosis; melanoma
Categories
Ask authors/readers for more resources
Dihydromyricetin (DHM), a Rattan tea extract, has recently been shown to have anti-cancer activity in mammalian cells. In this study, we investigated the effect of DHM on human melanoma cells. Apart from induction of apoptosis, we demonstrated that DHM induced an autophagic response. Moreover, pharmacological inhibition or genetic blockade of autophagy enhanced DHM-induced cell death and apoptosis, indicating the cytoprotective role of autophagy in DHM-treated human melanoma cells. Further study suggested that the nuclear factor kappa B (NF-kappa B) signalling pathway was involved in DHM-induced autophagy. Moreover, N-acetyl-cysteine (NAC), an ROS scavenger, abrogated the effects of DHM on NF-kappa B-dependent autophagy. Taken together, this evidence demonstrates that a strategy of blocking ROS-NF-kappa B-dependent autophagy to enhance the activity of DHM warrants further attention for the treatment of human melanoma.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available