Journal
FREE RADICAL BIOLOGY AND MEDICINE
Volume 107, Issue -, Pages 146-150Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.freeradbiomed.2016.11.025
Keywords
Oxidized abasic sites; 2-deoxyribonolactone; AP lyase; DNA polymerase beta; DNA polymerase gamma
Funding
- U.S. National Institutes of Health [R21CA198752, R21CA191856]
- U.S. National Aeronautics and Space Administration [NNA14AB04A]
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Oxygen is both necessary and dangerous for aerobic cell function. ATP is most efficiently made by the electrontransport chain, which requires oxygen as an electron acceptor. However, the presence of oxygen, and to some extent the respiratory chain itself, poses a danger to cellular components. Mitochondria, the sites of oxidative phosphorylation, have defense and repair pathways to cope with oxidative damage. For mitochondrial DNA, an essential pathway is base excision repair, which acts on a variety of small lesions. There are instances, however, in which attempted DNA repair results in more damage, such as the formation of a DNA-protein crosslink trapping the repair enzyme on the DNA. That is the case for mitochondrial DNA polymerase gamma acting on abasic sites oxidized at the 1-carbon of 2-deoxyribose. Such DNA-protein crosslinks presumably must be removed in order to restore function. In nuclear DNA, ubiquitylation of the crosslinked protein and digestion by the proteasome are essential first processing steps. How and whether such mechanisms operate on DNA-protein crosslinks in mitochondria remains to be seen.
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