Journal
CATALYSIS SCIENCE & TECHNOLOGY
Volume 11, Issue 5, Pages 1922-1930Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cy01915a
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Funding
- National Natural Science Foundation of China [31700078]
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This study successfully demonstrated the enzymatic bioconversion of d-fructose to d-mannose using a recombinant thermostable N-acyl-d-glucosamine 2-epimerase, resulting in high yields of d-mannose within 1 hour. The newly developed biocatalyst system shows potential for large-scale industrial production of d-mannose.
The synthesis of mannose is of great value because it is the only glyconutrient used for clinical applications. Here, we performed the enzymatic bioconversion of d-fructose to d-mannose using a recombinant thermostable N-acyl-d-glucosamine 2-epimerase (ThMI) from a Thermobifida halotolerans engineered strain. The enzymatic bioconversion produced 180 g L(-1)d-mannose from high-concentration d-fructose (500 g L-1) in 1 h by recombinant ThMI, resulting in a maximum bioconversion ratio of 35.8%. The recombinant resting cells at a density of 10 OD600 produced 157 g L(-1)d-mannose from 500 g L(-1)d-fructose in 1 h. These results suggest that the novel biocatalyst system developed in this study can be applied for large-scale industrial production of d-mannose.
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