Journal
THERAPEUTIC DELIVERY
Volume 12, Issue 4, Pages 287-296Publisher
FUTURE SCI LTD
DOI: 10.4155/tde-2020-0093
Keywords
adipocytes; antioxidants; N-acetylcysteine; nanoparticles
Categories
Funding
- Consejo Interuniversitario Nacional -fellowship
- Universidad de Buenos Aires [UBACYT 20020160100105BA]
- Universidad Nacional de Lujan [DISP CBLUJ 32-18]
Ask authors/readers for more resources
The study aimed to increase the cellular uptake of NAC by encapsulating it in silica nanoparticles, but the coated nanoparticles showed a reduced effect on Tg cellular accumulation.
Background: The addition of 5 mM N-acetylcysteine (NAC) to 3T3-L1 adipocytes culture inhibits the accumulation of triglycerides (Tg) by 50%, but after 48 h uptake was only 16% of total NAC available. Based on these results, the aim of this study is to increase the NAC cellular uptake by encapsulating it in silica nanoparticles (NPs). Materials & methods: Silica NPs, 20 +/- 4.5 nm in size, were developed, with an inner cavity loaded with 5 mM NAC. At 48 h after treatment, there was a dose-dependent cytotoxic effect. We attempted to reduce the cytotoxicity of silica NPs by coating them with bovine serum albumin. Results: While we obtained nontoxic bovine serum albumin coated NPs, their effect on Tg cellular accumulation was also reduced.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available