Journal
RSC ADVANCES
Volume 11, Issue 17, Pages 10054-10060Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0ra10602j
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Funding
- National Natural Science Foundation of China [NNSFC 61804158]
- Shanghai Sailing Program [18YF1428300]
- Program of Science and Technology Commission of Shanghai Municipality [17JC1401001]
- STS program [KFJ-STS-ZDTP-061]
- Shanghai Municipal Education Commission [18CG67]
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This study describes the development, verification, and practical application of an aptasensor for the fluorometric detection of kanamycin. The sensor has a detection limit of 0.1 pM and a detection range of 0.1 pM to 0.1 mu M, showing excellent performance in complex samples and providing obvious advantages over other analytical methods. The mechanism of action between gold nanoparticles/FAM-aptamer/kanamycin is discussed and studied in depth, offering more thorough analysis and application possibilities for fluorescence-aptamer biosensing.
This study describes the development, verification and practical application of an aptasensor for the fluorometric detection of kanamycin. Using the nucleic acid aptamer with FAM fluorescent group as the conjugate, using gold nanoparticles as the fluorescence dynamic quenching source, a fluorescence sensor was fabricated through the signal-on method for the micro-detection of kanamycin. The nucleic acid chimera is connected to the fluorophore, and the gold nanoparticles are used as the fluorescence dynamic quenching source under actual conditions. The detection limit of kanamycin is 0.1 pM, and the detection range is 0.1 pM to 0.1 mu M. This biosensor works satisfactorily in complex samples with no impurities, which gives this method an obvious advantage over other analytical methods. In addition, the mechanism of action between gold nanoparticles/FAM-aptamer/kanamycin is discussed and studied in depth here. It provides a more thorough analysis and more application possibilities for fluorescence-aptamer biosensing.
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