Journal
LAB ON A CHIP
Volume 21, Issue 5, Pages 962-975Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0lc01143f
Keywords
-
Ask authors/readers for more resources
The latest development in TFT-DMF technology, aQdrop (TM), shows promising applications in molecular diagnostics, including COVID-19 testing. With 41,000 independently addressable electrodes, TFT-DMF can manipulate droplets of any size and shape to perform multiple parallel reactions, demonstrating its potential for complex assays and sample processing.
The latest developments in thin-film-transistor digital-microfluidics (TFT-DMF, also known by the commercial name aQdrop (TM)) are reported, and proof of concept application to molecular diagnostics (e.g. for coronavirus disease, COVID-19) at the point-of-need demonstrated. The TFT-DMF array has 41 thousand independently addressable electrodes that are capable of manipulating large numbers of droplets of any size and shape, along any pathway to perform multiple parallel reactions. Droplets are continually tracked and adjusted through closed-loop feedback enabled by TFT based sensors at each array element. The sample-to-answer molecular in vitro diagnostic (IVD) test for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) includes nucleic acid extractions from saliva, removal of dsDNA and quantitative reverse transcription polymerase chain reaction (RT-PCR). This proof of concept illustrates how the highly configurable TFT-DMF technology can perform many reactions in parallel and thus support the processing of a range of sample types followed by multiple complex multi-step assays.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available