Journal
FORENSIC SCIENCE INTERNATIONAL-GENETICS
Volume 28, Issue -, Pages 21-34Publisher
ELSEVIER IRELAND LTD
DOI: 10.1016/j.fsigen.2017.01.005
Keywords
SWGDAM developmental validation; Rapid DNA RapidHIT (R); RapidHIT (R) ID; RapidLINK (TM); GlobalFiler (R) Express; STR; Presumed single source sample
Categories
Funding
- IntegenX
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The RapidHIT (R) ID is a fully automated sample-to-answer system for short tandem repeat (STR)-based human identification. The RapidHIT ID has been optimized for use in decentralized environments and processes presumed single source DNA samples, generating Combined DNA Index System (CODIS)compatible DNA profiles in less than 90 min. The system is easy to use, requiring less than one minute of hands-on time. Profiles are reviewed using centralized linking software, RapidLINK (TM) (IntegenX, Pleasanton, CA), a software tool designed to collate DNA profiles from single or multiple RapidHIT ID systems at different geographic locations. The RapidHIT ID has been designed to employ GlobalFiler (R) Express and AmpFLSTR (R) NGMSElect (TM), Thermo Fisher Scientific (Waltham, MA) STR chemistries. The Developmental Validation studies were performed using GlobalFiler (R) Express with single source reference samples according to Scientific Working Group for DNA Analysis Methods guidelines. These results show that multiple RapidHIT ID systems networked with RapidLINK software form a highly reliable system for wide-scale deployment in locations such as police booking stations and border crossings enabling real-time testing of arrestees, potential human trafficking victims, and other instances where rapid turnaround is essential. (C) 2017 The Author(s). Published by Elsevier Ireland Ltd.
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