Plastid phylogenomic insights into the evolution of subfamily Dialioideae (Leguminosae)

The subfamily Dialioideae (Leguminosae) consists of 17 genera and about 85 species. Previous studies have detected significant plastid genome (plastome) structure variations in legumes, particularly in subfamilies Papilionoideae and Caesalpinioideae. Hence it is important to investigate plastomes from the newly recognized Dialioideae to better understand the plastome variation across the whole family. Here, we used nine plastomes representing nine genera of Dialioideae to explore plastome structural variation and intergeneric relationships in this subfamily. All plastomes of Dialioideae exhibited a typical quadripartite structure, and had relatively conserved structure compared with other legume subfamilies. However, the genome size ranged from 154,124 bp to 165,973 bp and gene numbers ranged from 129 to 132, mainly due to the expansion and contraction of the inverted repeat (IR) regions. The IR of Distemonanthus benthamianus has experienced two separate expansions into the large single copy (LSC) region and the small single copy (SSC) region, and one contraction from SSC. Poeppigia procera has experienced two separate IR expansions into LSC, while Dicorynia paraensis has experienced an IR contraction from LSC. Highly divergent regions or genes (ndhC-trnV(UAC), psbK-trnQ(UUG), rps19-rps3, rpl33-rps18,accD-psaI,trnG(UCC)-trnS(GCU),psbI-trnS(GCU),5'rps16-trnQ(UUG) and ycf1) were identified as potential molecular markers for further species delimitation and population genetics analysis in legumes. Phylogenetic analysis based on 77 protein-coding sequences fully resolved the intergeneric relationships among nine genera except a moderately supported sister relationship between Petalostylis labicheoides and Labichea lanceolata. Our study reveals new insights into the structural variations of plastomes in subfamily Dialioideae and advances our understanding of the evolutionary trajectories of legume plastomes. Copyright (C) 2020 Kunming Institute of Botany, Chinese Academy of Sciences. Publishing services by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.

Automated summary

The subfamily Dialioideae of Leguminosae consisting of 17 genera and about 85 species shows conservative plastome structure, with variations in genome size and gene number mainly attributed to expansion and contraction of inverted repeat regions. Divergent regions or genes have been identified as potential molecular markers for further species delimitation and population genetics analysis in legumes. Phylogenetic analysis based on protein-coding sequences fully resolved intergeneric relationships among nine genera, providing new insights into plastome structural variations in Dialioideae and the evolutionary trajectories of legume plastomes.