Journal
THERANOSTICS
Volume 11, Issue 11, Pages 5174-5196Publisher
IVYSPRING INT PUBL
DOI: 10.7150/thno.56471
Keywords
Aptamer; lateral flow assay; point-of-care; biosensor; sandwich assay; competitive assay
Categories
Funding
- McCusker foundation through Perron Institute of Neurological and Translational Sciences
- Murdoch University
- Murdoch University commercial research funding
- Perron early and mid-career researcher internal grants scheme
- Department of Health Western Australia COVID-19 grant
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This study analyzes key issues in the design of aptamer-based LFAs, including immobilization strategies, signaling methods, and target capturing approaches, providing a comprehensive overview of aptamer-based LFA design strategies to facilitate the development of optimized aptamer-based LFAs by researchers.
Lateral flow assay (LFA) has made a paradigm shift in the in vitro diagnosis field due to its rapid turnaround time, ease of operation and exceptional affordability. Currently used LFAs predominantly use antibodies. However, the high inter-batch variations, error margin and storage requirements of the conventional antibody-based LFAs significantly impede its applications. The recent progress in aptamer technology provides an opportunity to combine the potential of aptamer and LFA towards building a promising platform for highly efficient point-of-care device development. Over the past decades, different forms of aptamer-based LFAs have been introduced for broad applications ranging from disease diagnosis, agricultural industry to environmental sciences, especially for the detection of antibody-inaccessible small molecules such as toxins and heavy metals. But commercial aptamer-based LFAs are still not used widely compared with antibodies. In this work, by analysing the key issues of aptamer-based LFA design, including immobilization strategies, signalling methods, and target capturing approaches, we provide a comprehensive overview about aptamer-based LFA design strategies to facilitate researchers to develop optimised aptamer-based LFAs.
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