Journal
FOOD RESEARCH INTERNATIONAL
Volume 100, Issue -, Pages 121-133Publisher
ELSEVIER
DOI: 10.1016/j.foodres.2017.06.018
Keywords
Sardinella aurita; Protein hydrolysate; Antibacterial; Anti-ACE; Antioxidant; Peptide
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Funding
- Ministry of Higher Education and Scientific Research-Tunisia
- MINECO (Spain) [AGL2014-57367-R]
- FEDER funds
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Sardinelle protein hydrolysate (SPH), prepared by treatment with Bacillus subtilis A26 proteases, was found to exhibit antibacterial, antioxidant and ACE-inhibitory activities. SPH, with a degree of hydrolysis of 4%, was fractionated by size exclusion chromatography on a Sephadex G-25 into five major fractions (F1-F5). F2, which exhibited the highest antibacterial and ACE-inhibitory activities, and F4, which exhibited the highest antibacterial and antioxidant activities, were further fractionated by reverse phase-high performance liquid chromatography (RP-HPLC) and then analysed using nano-ESI-LC-MS/MS to identify the sequences of peptides. Eight peptides were identified in the sub-fraction F2-A, nine peptides in the sub-fraction F4-B, and 45 peptides in F4-C. Identified peptides were found to share sequences with previously described bioactive peptides based on Biopep database. The results of this study suggest that SPH is a good source of natural bioactive peptides. Hence, it can be used as a potential ingredient in nutraceutical field.
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