Journal
FOOD RESEARCH INTERNATIONAL
Volume 100, Issue -, Pages 477-485Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.foodres.2016.11.001
Keywords
'Crossbreeding Chemlali by Zalmati'; Extra virgin olive oil; Phenolic compound HPLC-DAD-ESI-MS/MS; Principal component analysis
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Funding
- Ministere de I'Enseignement Superieur et de la Recherche Scientifique, Laboratory, Tunisia [LR14ES08]
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The aim of the present research is to evaluate the chemical characterization of main compounds from Tunisian monocultivar extra virgin olive oil (EVOO) ('Chemlali', 'Chetoui', 'Zalmati' and 'crossbreeding Chemlali by Zalmati') extracted after the addition of different amounts (0% and 3%) of olive leaves. As expected for extra virgin olive oil, the main sterols found in all analyzed samples were beta-sitosterol, Delta-5-avenasterol, campesterol and clerosterol. Stigmasterol, 24 -methylene-cholesterol, cholesterol, campestanol, sitostanol, Delta-7-stigmastenol, Delta-5,24-stigmastadienol, and Delta-7-avenasterol were also found in all samples, but in lower amounts. Most of these compounds were significantly affected by the cultivars but not to be affected by added leaves at 3% to olives prior to the extraction process. The obtained results revealed that 14 phenolic compounds belonging to different phenolic types were characterized and quantified by an effective HPLC-DAD-ESI-MS/MS method. In all the studied olive oil samples, dialdehydic form of elenolic acid linked to hydroxytyrosol (3,4-DHPEA-EDA), oleuropein aglycon (3,4-DHPEA-EA), and ligstroside aglycon (p-HPEA-EA) were the most abundant compounds. In addition, EVOO from 'Chetoui cultivar extracted with 3% of olive leaves presented the highest amount of individual phenolic compounds. (C) 2016 Elsevier Ltd. All rights reserved.
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