Journal
CROP JOURNAL
Volume 9, Issue 2, Pages 360-371Publisher
KEAI PUBLISHING LTD
DOI: 10.1016/j.cj.2020.08.010
Keywords
Direct-seeded rice; Early seedling vigor; QTL mapping; Expression profiling; Candidate genes
Categories
Funding
- Breeding New Varieties of Rice Suitable for Light and Simple Cultivation and Mechanized Production Project [2017YFD0100104]
- Research and Development Plan for Key Areas in Guangdong Province [2018B020206002]
- China Agriculture Research System [CARS-01-17]
- South China Agricultural University Doctoral Innovative Talents (Domestic Training) Cultivation Program [CX2019N044]
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This study identified stable quantitative trait loci (QTL) affecting early seedling vigor in rice, providing important genetic resources for rice breeding. Additionally, combining specific genes can effectively improve the performance of rice varieties.
Early seedling vigor (ESV) is a major breeding target in rice, especially under direct seeding. To identify quantitative trait locus (QTL) affecting ESV, a recombinant inbred line population derived from a cross between 02428 and YZX, two cultivars differing in vigor during early seedling growth, was used for QTL analysis. Nine traits associated with ESV were examined using a high-density map. Of 16 additive loci identified, three were detected in two generations and thus considered stable. Four epistatic interactions were detected, one of which was repeated in two generations. Further analysis of the pyramiding effect of the three stable QTL showed that the phenotypic value could be effectively improved with an increasing number of QTL. These results were combined with results from our previous QTL analysis of the germination index. The lines G58 and G182 combined all the favourable alleles of all three stable QTL for ESV and three QTL for germination speed. These two lines showed rapid germination and strong ESV. A total of 37 candidate differentially expressed genes were obtained from the regions of the three stable QTL by analysis of the dynamic transcriptomic expression profile during the seedling growth period of the two parents. The QTL are targets for ESV breeding and the candidate genes await functional validation. This study provides a theoretical basis and a genetic resource for the breeding of direct-seeded rice. (C) 2020 Crop Science Society of China and Institute of Crop Science, CAAS. Production and hosting by Elsevier B.V. on behalf of KeAi Communications Co., Ltd.
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