Journal
FOOD CONTROL
Volume 73, Issue -, Pages 1039-1044Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2016.10.019
Keywords
Clenbuterol; Salbutamol; Multi-assay; TRFIA; Water and swine feed samples
Categories
Funding
- Natural Science Foundation of China [31570414, 31502118]
- Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province [CX(13)3093]
- National Natural Science Foundation of Jiangsu Province [BK20140543, BK20130507, BK20130499]
- Foundation of State Key Laboratory of Environmental Chemistry and Ecotoxicology [KF2014-02]
- Senior Talent Scientific Research Initial Funding Project of Jiangsu University [16JDG035]
- Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD)
- Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment
Ask authors/readers for more resources
Lanthanide ions, the excellent fluorescence markers, have the potential to simplify the performance of multi-analytes analysis. In this work, a powerful time-resolved fluoroimmunoassay (TRFIA) based on dual-labeled format were performed for detecting clenbuterol and salbutamol simultaneously in water and swine feed samples. Europium (Eu3+)-labeled goat anti-mouse IgG and Samarium (Sm3+)-labeled goat anti-rabbit IgG were prepared and used as fluorescent probes. Under optimal conditions, the half maximal inhibition concentration (IC50) and the limit of detection (LOD, IC10) of the TRFIA were 0.263 and 0.007 ng/mL for clenbuterol, and 0.179 and 0.014 ng/mL for salbutamol, respectively. The cross-reactivities of the TRFIA between these two beta(2)-agonists and with their analogues were negligible, thus indicated high specificity and promoted the simultaneous determination. The spiked recoveries were 72.2%-102.2% with relative standard deviations (RSDs) of 3.4-13.8% for clenbuterol and salbutamol in water and swine feed samples. Furthermore, the results of TRFIA correlated well with those obtained by HPLC. The proposed dual -labeled TRFIA was a satisfactory tool for simultaneous, sensitive, efficient and labour-saving detection of clenbuterol and salbutamol in water and swine feed samples. (C) 2016 Elsevier Ltd. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available