Fluorescent sensor assay for β-lactamase in milk based on a combination of aptamer and graphene oxide

Fluorescent sensor assay (FSA) was developed by using a fluorescein-labeled aptamer assembled onto Graphene oxide (GO) in order to determine beta-lactamase in milk. Under optimal conditions, FSA indicated a detection range from 1 to 46 U/mL with a limit of detection (LOD) of 0.5 U/mL (R-2 = 0.999, n = 3). In addition, commercial milk samples tainted with beta-lactamase were detected by the established FSA with a recovery rate between 96.04 and 119.67%. Additionally, the reliability and sensitivity of FSA was validated by enzyme-linked immunoassay (ELISA) with a high correlation of 0.993. Thus, these data, combined with the ease and speed of the assay, suggest that the developed FSA may represent a promising method for monitoring beta-lactamase contamination in milk. (C) 2016 Elsevier Ltd. All rights reserved.