Journal
FOOD CONTROL
Volume 72, Issue -, Pages 62-72Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodcont.2016.07.031
Keywords
Dielectric barrier discharge; Inactivation; Non-thermal gas plasma; Oxidative stress; Xanthomonas campestris pv. campestris
Categories
Funding
- Bio-oriented Technology Research Advancement Institution (BRAIN)
- Science and Technology Research Promotion Program for Agriculture, Forestry, Fisheries and Food Industry [26015A]
- Amano Institute of Technology
- Uruma Fund for the Promotion of Science
- Takahashi Industrial and Economic Research Foundation
- Japan Society for the Promotion of Science [22110514, 24110717, 16K04997]
- Kieikai Research Foundation
- Grants-in-Aid for Scientific Research [24110717, 22110514, 24108009, 16K04997] Funding Source: KAKEN
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We have developed a novel roller conveyer plasma device, which generates gas plasma via atmospheric pressure dielectric barrier discharge (APDBP) on rollers. High voltage and earth electrodes are incorporated into the rollers, which are covered with a silicon sheet. Bactericidal activity of the device was measured against Xanthomonas campestris pv. campestris (Xcc), which was spotted onto aluminium plates and subjected to gas plasma treatment generated by APDBD. After treatment, viable cell number of Xcc decreased with a decimal reduction time (D value) of 0.90 min (initial population: 5.0 x 10(8) CFU/ml) and below 0.34 min (initial population: 9.8 x 10(5) CFU/ml). APDBD treatment induced slight morphological changes to Xcc along with significant degradation of lipopolysaccharides (LPS) and degradation/oxidation of genomic DNA to form 8-hydroxy-2'-deoxyguanosine (8-OHdG). Reactive chemical product hydrogen peroxide, nitrite and nitrate), ultraviolet light (UV-A) and slight temperature elevations were observed during operation of the device. Our results suggest hydrogen peroxide and nitrite generated during operation of the device cause oxidation and degradation of the genomic DNA as well as the degradation of LPS, which are potential mechanisms for inactivation. In addition, the successful inactivation of Xcc on cabbage leaf by the device suggests the method could have practical applications. (C) 2016 Elsevier Ltd. All rights reserved.
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