Journal
FOOD CHEMISTRY
Volume 232, Issue -, Pages 689-696Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.04.008
Keywords
Dillenia indica L.; Betulinic acid; RP-HPLC; Mushroom tyrosinase; Non-competitive inhibition; CD spectroscopy; In-silico analysis
Funding
- Department of Biotechnology, Government of India, New Delhi [BT/HRD/35/01/04/2014]
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The fruit of Dillenia indica L. is extensively used as a food additive. Betulinic acid (BA) is the most prominent secondary metabolite present in D. indica. This study screened the bioassay guided isolation of BA from D. indica and explored its tyrosinase inhibitory mechanism. Half maximal inhibitory concentration (IC50) of BA were calculated as 13.93 mu M and 25.66 mu M for diphenolase and monophenolase. Enzyme kinetic analysis revealed that BA inhibited tyrosinase activity non-competitively. Further, conformational analysis of tyrosinase with BA was measured by fluorescence and circular dichroism spectroscopy. These results implied that diminish rigidity of enzyme might disturb the catalytic conformation of tyrosinase. Moreover, In-silico analysis confirmed probable binding polar and non-polar region on the active site of tyrosinase. Based on these findings, we suggest that BA from D. indica may be useful in preventing enzymatic browning reactions in food products. (C) 2017 Elsevier Ltd. All rights reserved.
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