Journal
CATALYSIS SCIENCE & TECHNOLOGY
Volume 11, Issue 6, Pages 2186-2194Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d0cy02397c
Keywords
-
Categories
Funding
- Department of Biotechnology, Govt. of India
- DBT, GoI
- BIRAC, GoI
Ask authors/readers for more resources
This study successfully synthesized a high value rare sugar d-tagatose using recyclable magnetic catalysts and developed two novel magnetic cross-linked enzyme aggregates for efficient transformation of d-galactose and lactose. The immobilized catalysts showed high catalytic activity and reusability potential, indicating promising catalytic potential for the synthesis of d-tagatose.
In this study, a high value rare sugar d-tagatose was synthesized using recyclable magnetic catalysts. A magnetic cross-linked enzyme aggregate (mCLEA) of l-arabinose isomerase (l-AI) was developed for the transformation of d-galactose into d-tagatose. Similarly, a combined magnetic cross-linked enzyme aggregate (Combi-mCLEA) of dual enzyme l-AI and beta-galactosidase (beta-Gal) was also prepared for the synthesis of d-tagatose from lactose in a cascade reaction. To accomplish this, l-AI alone and together with beta-Gal was stabilized in magnetic nanoparticles (MNPs) and precipitated by an appropriate precipitating agent and then cross-linked by glutaraldehyde under optimal conditions. Immobilized catalysts were characterized by scanning electron microscopy, confocal laser scanning microscopy, Fourier transform infrared spectroscopy and thermogravimetric analysis. Results suggested that the immobilized catalysts retained a maximum initial activity and achieved equilibrium level conversion in 24 h of enzyme catalysis. The main advantage of the developed magnetic catalysts was their recovery using an external magnet after completion of the reaction. Additionally, mCLEA and Combi-mCLEA showed reusability potential for more than 10 consecutive batch cycles of rare sugar d-tagatose synthesis from d-galactose and lactose, respectively. Thus, these immobilized magnetic catalysts have shown promising catalytic potential for the synthesis of d-tagatose.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available