Journal
FOOD CHEMISTRY
Volume 218, Issue -, Pages 321-329Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.09.072
Keywords
Anti-nutritional factor; Environment; Genotypes; Soybean; 2D-PAGE; MALDI-TOF-MS; Proteomics
Funding
- ARS [8042-21220-232-00D]
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The genotype (G), environment (E), and the relationship between G and E on soybean seed anti-nutritional factors (ANF's) were examined under three different agro-climatic conditions. The field trials were conducted at Maryland, South Carolina and South Dakota using nine region specific genotypes. At each location, the nine genotypes were grown with two planting/sowing dates. Differentially expressed protein spots from the two-dimensional gel electrophoresis were analyzed using mass spectrometry. Seven ANF's corresponding to soybean agglutinin and Kunitz trypsin inhibitor were identified based on the statistical significance levels at p < 0.005. The G and E conditions (planting/sowing season) influences the ANF's content. This initial study suggests that early sowing reduces the total ANF's content irrespective of genotypes and their growing locations. Published by Elsevier Ltd.
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