Journal
FOOD CHEMISTRY
Volume 232, Issue -, Pages 770-776Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.04.058
Keywords
Amantadine; Immunochromatography; Semi-quantitative analysis; Chicken muscle
Funding
- Jiangxi Special Fund for Agro-Scientific Research in the Collaborative Innovation [JXXTCX2017003]
- Jiangxi Graduate Innovative Special Fund Projects
- Jiangxi Agriculture Research System [JXARS-03]
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Amantadine (AMD) is a prohibitive veterinary medicine in the entire world. In this study, a sensitive colloidal gold immunochromatographic assay (CGICA) was established for the rapid semi-quantitative detection of AMD in chicken muscle. Under optimal conditions, the detection results were obtained in 12 min with a limit of detection for 1.80 ng/mL. CGICA presented a good linear range from 2.5 ng/mL to 25 ng/mL, with only 11.5% cross-reactivity with rimantadine. The recovery rates for the fortified samples were ranged from 81% to 120%. The coefficient of variation of the intra-assay and inter-assay was less than 15%. The accuracy of CGICA was confirmed by systematically comparing the result of the proposed method with enzyme-linked immunosorbent assay and ultra-high-performance liquid chromatography-tandem mass spectrometry. Given the advantages of its simplicity, convenience, and speediness, the proposed CGICA is suitable for the on-site rapid detection of AMD in chicken muscle. (C) 2017 Elsevier Ltd. All rights reserved.
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