Agarose-chitosan-C18 film micro-solid phase extraction combined with high performance liquid chromatography for the determination of phenanthrene and pyrene in chrysanthemum tea samples

Agarose-chitosan-immobilized octadecylsilyl-silica (C-18) film micro-solid phase extraction (mu SPE) was developed and applied for the determination of phenanthrene (PHE) and pyrene (PYR) in chrysanthemum tea samples using high performance liquid chromatography-ultraviolet detection (HPLC-UV). The film of blended agarose and chitosan allows good dispersion of C-18, prevents the leaching of C18 during application and enhances the film mechanical stability. Important mu SPE parameters were optimized including amount of sorbent loading, extraction time, desorption solvent and desorption time. The matrix match calibration curves showed good linearity (r >= 0.994) over a concentration range of 1-500 ppb. Under the optimized conditions, the proposed method showed good limits of detection (0.549-0.673 ppb), good analyte recoveries (100.8-105.99%) and good reproducibilities (RSDs <= 13.53%, n = 3) with preconcentration factors of 4 and 72 for PHE and PYR, respectively. (C) 2016 Elsevier Ltd. All rights reserved.