Journal
FOOD CHEMISTRY
Volume 219, Issue -, Pages 364-372Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2016.09.159
Keywords
Coffee; Antioxidant; Cytoprotective/cytotoxic; Caco-2 cells; Furan compounds
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Antioxidant capacity (AC), total phenolics (TPs), furan compounds (HMF and furfural F) and cytoprotective/cytotoxic effects upon Caco-2 cells (MTT, cell cycle and reactive oxygen species (ROS)) Were evaluated in Colombian coffee (2 ground and 4 soluble samples). The AC (ABTS and FRAP), TPs and HMF ranged between 124-722, 95-802 moles Trolox/g, 21-100 mg gallic acid/g and 69-2900 mg/kg, respectively. Pretreatment of cells for 24 h with lyophilized coffee infusions at the highest dose without cytotoxic effects (500 mu g/mL) significantly prevented the decrease in cell viability compared to control stress with H2O2 (5 mM/2 h), recovering viability to values between 34% and 45% and restoring the control values without stress induction in the G1 phase of cell cycle. After exposure to stress, four extracts decreased ROS values significantly to 22.5-24.9%. The coffee samples exerted a cytoprotective effect against oxidative stress, with improvement in cell viability and a reduction of intracellular ROS. (C) 2016 Elsevier Ltd. All rights reserved.
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