Journal
NEW JOURNAL OF CHEMISTRY
Volume 45, Issue 12, Pages 5322-5326Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/d1nj00036e
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Funding
- Science and Technology Project of Ji Hua Laboratory [X190191TD190]
- Training Program of Gusu Health Talents [GSWS2019023]
- Medical Health Science and Technology Project of Suzhou New District [2019Z015]
- Science and Technology Program of Suzhou [SS2019077]
- Collaborative Innovation Program of Jinan [2018GXRC033]
- National Natural Science Foundation of China [61805273]
- Natural Science Foundation of Jiangsu Province [BK20170389]
- Youth Innovation Promotion Association of the Chinese Academy of Sciences [2017374]
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A novel colorimetric method for detecting telomerase activity based on the cyclic strand displacement reaction is proposed, which shows good sensitivity and provides a new approach for early cancer diagnosis. The method evaluates telomerase activity by disrupting the protection of gold nanoparticles from salt-induced aggregation.
In this work, a novel colorimetric method for the detection of telomerase activity is proposed based on the cyclic strand displacement reaction. Target telomerase firstly generates an extension product, which displaces a single-stranded DNA from a partial DNA duplex. The released DNA probe further hybridizes with another DNA probe and initiates the cyclic strand displacement reaction coupling two hairpin DNA probes. The consumption of these DNA probes destroys the protection of gold nanoparticles (AuNPs) from salt-induced aggregation. By observing the color or measuring the UV-vis adsorption spectra, the level of telomerase activity can be evaluated. This method exhibits good sensitivity and provides a new method for telomerase assay for early diagnosis of cancers.
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