Metabolite Profiles of Agarwood Gyrinops versteegii (Gilg) Domke Leaves collected from Different Locations

The leaves of Gyrinops versteegii, an agarwood-producing plant, are reported to have many benefits, including antioxidant, anti-malaria and an anti-proliferation activity against several cancer cell lines. The plants originate from the Eastern part of Indonesia and are now cultivated for resin production in several parts of Indonesia. Secondary metabolites are known to be highly influenced by environmental factors. This study was conducted to evaluate the effect of different planting locations on the leave metabolite profiles of G. versteegii. Leaf samples were collected from two different locations, Bogor Botanical Gardens, West Java and Research and Development Institute of Technology Non-Timber Forest Product Mataram, Lombok Island. Samples were extracted by maceration overnight with non polar to polar solvents of chloroform, ethyl acetate and ethanol at a ratio of 1:13 (w/v). The extracts were air-dried and then analyzed using GC-MS. The chromatograms were further processed for confirmation of the metabolites content. The metabolite profiles were compared using multivariate analysis in SIMCA P software and significant differences were confirmed with a t-test. The Bogor sample's metabolite profile was different from the Mataram sample, specifically fifteen compounds including fatty acids, monoterpenes, sesquiterpenes and triterpenes. This difference may be due to the different soil composition between the two locations. The soil in Bogor had higher concentrations of K and Mn, but lower N and Ca compared to the Mataram soil. The different location was confirmed by the metabolite profiles of agarwood leaves and the G. versteegii leaves from Bogor are different from those from Mataram.

Automated summary

This study evaluated the impact of different planting locations on the leaf metabolite profiles of G. versteegii, finding that the soil composition of different locations can influence the metabolite composition of leaves.