Journal
FOOD CHEMISTRY
Volume 230, Issue -, Pages 49-57Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.03.015
Keywords
DNA barcode; COI; Cytb; High resolution melting analysis; Real-time PCR; Codfish; Gadoid species
Funding
- FCT (Fundacao para a Ciencia e Tecnologia) [UID/QUI/50006/2013 - POCI/01/0145/FEDER/007265]
- FCT/MEC through national funds
- FEDER [NORTE-01-0145-FEDER-000011]
- FCT [SFRH/BD/93711/2013, SFRH/BPD/102404/2014]
- POPH-QREN
- FSE
- MCTES
- Fundação para a Ciência e a Tecnologia [SFRH/BD/93711/2013] Funding Source: FCT
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This work aimed to exploit the use of DNA mini-barcodes combined with high resolution melting (HRM) for the authentication of gadoid species: Atlantic cod (Gadus morhua), Pacific cod (Gadus macrocephalus), Alaska pollock (Theragra chalcogramma) and saithe (Pollachius virens). Two DNA barcode regions, namely cytochrome c oxidase subunit I (COI) and cytochrome b (cytb), were analysed in silico to identify genetic variability among the four species and used, subsequently, to develop a real-time PCR method coupled with HRM analysis. The cytb mini-barcode enabled best discrimination of the target species with a high level of confidence (99.3%). The approach was applied successfully to identify gadoid species in 30 fish containing foods, 30% of which were not as declared on the label. Herein, a novel approach for rapid, simple and cost-effective discrimination/clustering, as a tool to authenticate Gadidae fish species, according to their genetic relationship, is proposed. (C) 2017 Elsevier Ltd. All rights reserved.
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