Journal
AGING-US
Volume 13, Issue 6, Pages 8335-8354Publisher
IMPACT JOURNALS LLC
Keywords
HSC; transdifferentiation; pneumonocytes; radiation
Categories
Funding
- National Natural Science Foundation of China [81673093, 31201073, 82000117, 91649113, 81800152, 31771640, 81570126]
- Jiangsu Science and Technology Department [BK20130333, BK20160330, BK20200191]
- Postdoctor Science Foundation of Jiangsu Province [2020Z064]
- Priority Academic Program Development of Jiangsu Higher Education Institutions
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The study found that hematopoietic stem cell (HSC) transplantation can repair radiation-induced lung tissue damage, with some HSCs potentially transdifferentiating into pneumonocytes. These data suggest that donor HSCs may migrate to the injured lung of the recipient, repairing the damage caused by radiation.
Accumulative radiation exposure leads to hematopoietic or tissue aging. Whether hematopoietic stem cells (HSCs) are involved in lung damage repair in response to radiation remains controversial. The aim of this study is to identify if HSC can transdifferentiate to pneumonocytes for radiation-induced damage repair. To this end, HSCs from male Rosa(mT/mG) mice were isolated by fluorescence-activated cell sorting (FACS) and transplanted into lethally irradiated female CD45.1 mice. 4 months after transplantation, transplanted HSC was shown to repair the radiation-induced tissue damage, and donor-derived tdTomato (phycoerythrin, PE) red fluorescence cells and Ddx3y representing Y chromosome were detected exclusively in female recipient lung epithelial and endothelial cells. Co-localization of donor-derived cells and recipient lung tissue cells were observed by laser confocal microscopy and image flow cytometry. Furthermore, the results showed HSC transplantation replenished radiation-induced lung HSC depletion and the PE positive repaired lung epithelial cells were identified as donor HSC origin. The above data suggest that donor HSC may migrate to the injured lung of the recipient and some of them can be transdifferentiated to pneumonocytes to repair the injury caused by radiation.
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