Journal
FOOD CHEMISTRY
Volume 229, Issue -, Pages 761-768Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.foodchem.2017.03.001
Keywords
Sucrose isomerase; Heterologous expression; Food safety; Cane molasses; Isomaltulose; Biologic membrane reactor
Funding
- Natural Science Foundation of Jiangsu Education Ministry [14KJB530005]
- Jiangsu Province science and technology support plan project [BE2015366]
- state key laboratory of materials-oriented chemical engineering [ZK201403]
- Six Talent Peaks Project in Jiangsu Province [SWYY-027]
- Scientific Research and Innovation Program for General Universities in Jiangsu Province [KYLX16_0617]
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A green process and environmentally benign process is highly desirable in the development of enzymatic catalysis. In this work, the shuttle plasmid pHA01 was constructed and the sucrose isomerase (Slase) was expressed in Bacillus subtilis WB800. The optimal nitrogen and carbon sources for Slase expression were yeast extract (15 g/L) and un-pretreated cane molasses (UCM, 20 g/L), respectively. After the UCM fed, the whole cell activity reached 5.2 U/mL in a 7.5 L fermentor. Optimum catalytic temperature and pH of whole cell were 35 degrees C and 5.5, respectively. Although the biologic membrane reactor (BMR) system consecutively worked for 12 batches, the sucrose conversion remained higher than 90%, indicating the BMR system had a greater operational stability. Furthermore, isomaltulose production using the BMR system with low-cost cane molasses as its substrate not only reduces the production cost and mediates environmental pollution, but also solves the genetic background problem of the non-food-grade strains. (C) 2017 Elsevier Ltd. All rights reserved.
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