Milk protein isolate (MPI) as a source of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides

A multifactorial [temperature (40, 50 and 60 degrees C), hydrolysis time (60, 150 and 240 min) and enzyme to substrate ratio (E: S; 1.0, 1.5 and 2.0%)] design of experiments (DOE) was used to optimise the release of dipeptidyl peptidase IV (DPP-IV) inhibitory peptides during hydrolysis of bovine milk protein isolate (MPI) with Neutrase 0.8L (TM), yielding 15 hydrolysates (H1-H15). Variation in temperature and time had a significant effect on DPP-IV inhibitory properties (p < 0.05) in contrast with E: S (p > 0.05). The DPP-IV half maximal inhibitory concentration (IC50) of H4, a potent sample, was maintained following simulated gastrointestinal digestion (SGID, DPP-IV IC50 = 0.60 +/- 0.06 vs. 0.58 +/- 0.09 mg ml(-1), p > 0.05). Several peptides with DPP-IV inhibitory features or known activity were identified by liquid chromatographytandem mass spectrometry (LC-MS/MS) within the hydrolysates. MPI hydrolysates may have potential for use as dietary ingredients with serum glucose lowering activity in humans. (C) 2017 Elsevier Ltd. All rights reserved.