Journal
IEEE TRANSACTIONS ON MEDICAL ROBOTICS AND BIONICS
Volume 3, Issue 2, Pages 328-336Publisher
IEEE-INST ELECTRICAL ELECTRONICS ENGINEERS INC
DOI: 10.1109/TMRB.2021.3076210
Keywords
3D printing; organ-on-chip; extracellular matrix; kidney model; perfusable in-vitro model; bioartificial organ
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This paper describes the design of a chip modeling the proximal renal tubule, including a 3D convoluted geometry and a perfusion system. Various formulations were investigated, with optimal results found using a matrix composed mainly of Matrigel and Collagen I.
This paper describes the design of a chip modeling the proximal renal tubule, namely the nephron segment that is most targeted by drug toxicants. The tubule model is based on a 3D convoluted geometry whose printing parameters and Pluronic concentration were optimized to achieve a diameter of 320 mu m without discontinuities. A perfusion system guaranteeing a 1 mu L/min flow was also integrated. Moreover, the tubule model was integrated into a gel recapitulating the composition and the elastic properties of the human kidney extracellular matrix. Different formulations containing Matrigel, Collagen I and transglutaminase were investigated and compared in terms of rheometric properties and renal cell viability and ability to form a compact layer on them. Optimal results were found with a matrix composed of Matrigel and Collagen I (especially in the ratios 1:1), with the addition of 2.7% transglutaminase and 0.9% CaCl2.
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